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KMID : 0613820090190081125
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Journal of Life Science
2009 Volume.19 No. 8 p.1125 ~ p.1131
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Purification and Identification of Phenol Compounds with Inhibitory Activity on Helicobacter pylori from Rhododendron mucronulatum Flos. Extracts
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Ju In-Sik
Cho Young-Je
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Abstract
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The antimicrobial activities of Helicobacter pylori as a functional food source with water and 60% ethanol extracts from Rododendron mucronulatum Turcz. flowers were examined. The total phenol content of 60% ethanol extracts (30.6±0.14 ㎎/g) from Rododendron mucronulatum Turcz. flowers was higher than that of water extracts (23.2±0.21 ㎎/g). The inhibitory activities of Rododendron mucronulatum Turcz. extracts on H. pylori was determined to clear zone of 15 ㎜ in 80% ethanol extracts. Purification of inhibitory compounds was carried out in Sephadex LH-20 and MCI-gel CHP-20 column chromatography using a gradient procedure, with increasing ethanol(0→100%) in H₂O. The chemical structure of the purified inhibitory compounds of H. pylori was identified to be quercitrin (quercetin-3-O- rhamnopyranoside), myricitrin (myricetin-3-O-rhamnopyranoside), quercetin by FAB-MS, NMR and IR spectra.
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KEYWORD
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Antimicrobial compounds, Rododendron mucronulatum Turcz., Helicobacter pylori, purification
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